Probing Structurally Altered and Aggregated States of Therapeutically Relevant Proteins using GroEL Coupled to Bio-Layer Interferometry

Naik S, et al., doi: 10.1002/pro.2515, Protein Sci, 2014

The authors described the functional analysis and characterization of several pharmaceutically relevant proteins used as models to test a novel methodology utilizing the protein chaperonin GroEL coupled with Bio-layer interferometery to detect structurally altered and preaggregate/aggregate formation in protein solutions during slightly elevated to moderate thermal stress conditions. Understanding and possibly controlling protein aggregation events during the development processes can be key to successful therapeutic generation and accurate characterization. Binding studies were performed on the Blitz system using Streptavidin biosensors.

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