Predicting the Uncertain Future of Aptamer-Based Diagnostics and Therapeutics

Bruno J, 20(4):6866-87, Molecules, 2015

This is a review article elaborating the prospects of aptamers as diagnostics and therapeutic agents. The so-called conformational changes of a ligand upon analyte binding could cause a blue shift (instead of a red shift) and yield inverted binding curves on the Octet. The kinetic characterization and data analysis of a binding event involving an aptamer and a peptide is described. The author describes this observation as an induced fit binding mechanism pertaining to longer aptamers.

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