Predicting the Origins of Anti-blood Group Antibody Specificity: A Case Study of the ABO A- and B-antigens

Makeneni S, et al., doi: 10.3389/fimmu.2014.00397, Front. Immunol., 2014

The authors performed detailed structural and functional analysis on a monoclonal antibody raised against blood group A (BGA) antigen which complexes with scFv AC1001 in order to better understand the origin of antigenicity (the specificity and affinity) in ABO blood typing. Computer and theoretical modeling, crystallization studies, array screening and function binding analysis were employed to better understand the origin of antibody-carbohydrate specificity. Binding studies were performed on the Octet RED96 system using Amine Reactive Second-Generation (AR2G) biosensors.

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