Performance Characteristics of Monoclonal Antibodies as Recyclable Binders to Cardiac Troponin I

D.H. Kim, et al., 431(1), 11-8, Analytical Biochemistry, 2012

A method was developed to screen for rapidly reversible antibodies from 22 hybridoma clones against cardiac troponin 1 (cTn1). Antibodies were characterized based on association and dissociation kinetics using the Octet RED system and APS biosensors. A rapidly dissociating antibody was selected and shown to be recyclable. This antibody was used to continuously monitor analyte concentration by recycling the antibody as capture agent on a microfluidic sensor.

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