Peptide Ligands for Pro-survival Protein Bfl-1 from Computationally Guided Library Screening

Dutta, S, Chen, T S, Keating, A E, 8(4), 778-88 , ACS Chem Biol, 2013

The focus of this study was the protein Bfl-1, a pro-survival member of the Bcl-2 family implicated in a variety of cancers. The authors isolated a high affinity peptide that bound to Bfl-1 in preference to other pro-survival proteins, and performed mutational and structural analysis to identify positions important to preferential binding. The BLItz system was used to generate equilibrium and kinetic binding data for peptide-protein interactions. Biotinylated peptide was loaded onto Streptavidin biosensors for these measurements.

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