PA Tag: A Versatile Protein Tagging System Using a Super High Affinity Antibody Against a Dodecapeptide Derived from Human Podoplanin

Fujii Y, et al., 95:240-7, Protein Expr Purif, 2014

The authors describe the development of a novel affinity tagging system utilizing a unique mAb NZ-1 that is directed against human podoplanin and has an unusually high affinity toward linear dodecapeptide antigen designated "PA". The system has the abililty to purify low abundant proteins at high yield in one step along with its ability for extended reusability, therefore providing new opportunities for protein purification in many biomedical research areas. Binding kinetics were determined on the Octet RED system using Amine Reactive (AR2G) biosensors.

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