p53/HMGB1 Complexes Regulate Autophagy and Apoptosis

Livesey, K.M., et al., 72(8), 1996-2005, Cancer Research, 2012

Direct interaction of HMGB1 and p53 was evaluated in a cell-free system using the Octet QK system. Recombinant HMGB1 was immobilized onto Amine Reactive and Streptavidin biosensors; association and dissociation curves to recombinant p53 were generated to determine the affinity constant. KD values of the reverse orientations were very similar on both the sensors. Oxidation of HMGB1 had minimal effect whereas reduction of p53 abrogated interaction with HMGB1.

Read More