Noncanonical G Recognition Mediates KSRP Regulation of Let-7 Biogenesis

Nicastro, G., et al., 19(12), 1282-6, Nat Struct Mol Biol, 2012

The authors determine the contribution of individual KH domains of the protein KSRP to its interaction with let-7, a tumor-suppressive micro RNA. The Octet platform and Streptavidin biosensors were utilized to assess binding of KSRP mutants to let-7, followed by NMR structural studies to dissect the binding mechanism of KSRP KH domains to the G-rich target sequence on the terminal loop of let-7.

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