Nonbinding Site-Directed Mutants of Transferrin Binding Protein B Exhibit Enhanced Immunogenicity and Protective Capabilities

Frandoloso R, et al., 83(3):1030-8, Infect Immun, 2015

Described herein is the effect of binding host transferrin (Tf) on the immunogenicity of Tf binding proteins. A mutant Haemophilus parasuis Tf binding protein B (TbpB) demonstrated an improved B-cell and T-cell response in pigs compared to native TbpB. The dissociation constant (KD) of wild-type and mutant TbpBs was determined by Bio-Layer Interferometry (BLI). A Pall ForteBio Octet RED instrument equipped with Streptavidin Biosensor probes and immobilized with pTf was used in these experiments. A dilution series of TbpB was used for the determination of binding between TbpB-pTf . Overall findings are expected to influence the engineering of new vaccines against pathogens in the future.

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