New LIC Vectors for Production of Proteins from Genes Containing Rare Codons

Eschenfeldt WH, et al., 14, 135-144, J Struct Funct Genomics, 2013

The authors describe modification of a series of LIC pMCSG vectors currently used in the high-throughput production of proteins to include tRNA genes covering rare codons for Arg and Ile. Ionosine 5'-monophosphate dehydrogenase from B. anthracis was used as a test protein for the biotinylated vector system. Analyses of the binding of inhibitors to IMPDH proteins were performed using the Octet RED system with biotinylated IMPDH loaded onto Super Streptavidin biosensors.

Read More