Neutralizing and Non-neutralizing Monoclonal Antibodies Against Dengue Virus E Protein Dervied from a Naturally Infected Patient

Schieffelin, J.S., et al., 7, 28-38, Virology Journal, 2010

The authors generate human monoclonal antibodies (mAbs) against dengue virus envelope glycoprotein (E protein), and then characterize mAb binding and netralization. Kinetic binding assays between purified mAbs and purified E proteins were performed on the Octet QK system. E proteins were biotinylated, immobilized on Streptavidin biosensors, and incubated with mAb solutions to determine association and dissociation rate constants.

Read More