Nck-mediated Recruitment of BCAP to the BCR Regulates the PI(3)K-Akt Pathway in B Cells

Castello A, et al., 14(9), 966-75, Nat Immunol, 2013

This report describes a previously unknown adaptor function for Nck in recruiting BCAP to sites of BCR signaling and thereby modulating the PI(3)K-Akt pathway in B cells. The Octet RED96 system was used to characterize interactions of various recombinant proteins with an immunoglobulin-α 22-residue peptide centered on phosphorylated Tyr204. The biotinylated peptide was loaded onto Streptavidin biosensors for these experiments.

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