Mycobacterium tuberculosis RpfE Promotes Simultaneous Th1- and Th17-Type T-cell Immunity via TLR4-Dependent Maturation of Dendritic Cells

Choi H, et al., 45(7):1957-71, Eur J Immunol, 2015

Described herein is the role of resuscitation-promoting factor (Rpf) E in the host immune response against Mycobacterium tuberculosis (Mtb) infection. RpfE stimulates the Th1 and Th17 immune responses via maturation of dendritic cells, in a TLR4-dependent manner. A Pall ForteBio BLItz instrument equipped with a HIS Biosensor probe was used to study the binding of recombinant human TLR4/MD2 or TLR4 itself to RpfE. Both TLR4/MD2 complex and TLR4 alone were HIS-tagged for these experiments. Overall findings of this investigation indicate that the RpfE protein could be used to develop more effective multi-antigenic Mtb subunit vaccines.

Read More