Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity

Sun S, et al., 135(27), 9980-9983, J Am Chem Soc, 2013

The authors analyzed the effects of individual somatic mutations and combinations thereof on the antibody binding affinity and thermal stability of the well-characterized monoclonal antibody 48G7. Hapten binding affinities for 48G7 and mutants were studies using the Octet RED96 system. Biotinylated JWJ1 hapten was immobilization on Streptavidin biosensors for the binding studies.

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