Publications

Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity

Sun S, et al., 135(27), 9980-9983, J Am Chem Soc, 2013

The authors analyzed the effects of individual somatic mutations and combinations thereof on the antibody binding affinity and thermal stability of the well-characterized monoclonal antibody 48G7. Hapten binding affinities for 48G7 and mutants were studies using the Octet RED96 system. Biotinylated JWJ1 hapten was immobilization on Streptavidin biosensors for the binding studies.

Read More