Multiplexed Screening of Natural Humoral Immunity Identifies Antibodies at Fine Specificity for Complex and Dynamic Viral Targets

McCutcheon KM, et al., 6(2):460-73, Mabs, 2014

The authors performed a high throughput multiplexed antigen screen of memory B cell from immune individuals to identify potential antibodies that have a neutralizing effect on viral epitopes. The report describes optimization of the screening method coupled with in vivo experiments as a direction to better design of optimal vaccine antigens. Kinetic binding and affinity studies on recombinant antibodies were performed on the Octet QK system using Human IgG FC capture (AHC) biosensors.

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