Monitoring the Kinetics of the pH Driven Transition of the Anthrax Toxin Prepore to the Pore by Biolayer Interferometry and Surface Plasmon Resonance

Naik S, et al., 52, 6335-6347, Biochemistry, 2013

In this comprehensive study, the authors construct immobilized complexes of anthrax toxin prepore and the anthrax protective antigen (PA) binding domain of anthrax lethal factor to monitor the prepore-to-pore kinetic transition in real time, using both BLI and SPR. For BLI experiments, Octet RED96 and BLItz systems were used. Immobilization of PA was achieved via noncovalent binding to sulfhydryl-linked LFnE126C.

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