Molecular Determinants of the Interaction Between Doa1 and Hse1 Involved in Endosomal Sorting

Han S, et al., 446(1):352-7, Biochem Biophys Res Commun , 2014

The authors performed structural and functional analysis on the yeast protein Doa1, which has a role in endosomal sorting via an association with Hse1, a component of the endosomal sorting complex required for transport (ESCRT) system. Studies demonstrated that hydrogen binding is a major determinant in the interaction of these two proteins. Binding association was performed using a Blitz system along with Ni-NTA biosensors.

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