Modulation of the Binding of Basic Fibroblast Growth Factor and Heparanase Activity by Purified λ-Carrageenan Oligosaccharides

Niu T, eta;., 125:76-84, Carbohydr Polym, 2015

A group of seven λ-Carrageenan oligosaccharides (λ-COs) with different molecular weights were investigated in the present study for their ability to bind to basic fibroblast growth factor (bFGF) and to affect the activity of bFGF and heparanase, and their cell invasion characteristics. λ-COs are heavily-sulfated oligosaccharides with demonstrated ability to act as bFGF antagonists and as heparanase inhibitors. The interaction between λ-COs and bFGF was determined by a Pall ForteBio Octet system equipped with super Streptavidin biosensors. Recombinant bFGF was biotinylated and captured onto super Streptavidin biosensors and different analyte concentrations of λ-COs were screened for their ability to interact with bFGF.

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