Modeling Taylor Dispersion Injections: Determination of Kinetic/Affinity Interaction Constants and Diffusion Coefficients in Label-Free Biosensing

Quinn JG, 421: 391-401, Anal biochem, 2012

Standard fixed concentration injections (FCIs) are commonly used when studying biomolecular interactions by label-free detection methods such as surface plasmon resonance (SPR). With the FCIs, a serial dilution of the analyte is prepared and sequentially injected in order to obtain an analyte concentration range to determine kinetic parameters. Where as in Taylor dispersion injections (TDis), the analyte concentration gradually evolves into a sigmoidal gradient profile enabling kinetic analysis of biomolecular interactions from a minimal number of injections. Pall ForteBio Pioneer instruments incorporate a capillary tube that enables TDi assay formats. Reported herein is the use of a numerical model, Monte Carlo simulations, and statistical profiling in a comparison of Taylor dispersion injection approach with the standard fixed concentration injections approach. Overall results of this study demonstrates the effectiveness of the TDi approach.

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