Mechanisms Mediating Enhanced Neutralization Efficacy of Staphylococcal Enterotoxin B by Combinations of Monoclonal Antibodies

Dutta K, et al., 13;290(11):6715-30, J Biol Chem , 2015

Reported in this article are two different mechanisms of how a combination of different mAbs can enhance efficacy of mAb-mediated protection against Staphylococcal enterotoxin B (SEB). Combining two SEB-specific mAbs enhanced the overall efficacy, even though one of the two mAbs alone has no effect. A Pall ForteBio BLItz instrument equipped with a Streptavidin Biosensor probe was used to perform affinity determination experiments and competitive binding assays involving TCR- β chain, MHC-II, and the mAbs toward SEB. Biotinylated SEB was captured onto the biosensor tip. Understanding the mechanisms of how different combinations of mAbs enhance toxin neutralization is important to the development of new high efficacy antibody cocktails.

Read More