Maturation of the Proteasome Core Particle Induces an Affinity Switch That Controls Regulatory Particle Association

Wani P, et al., doi: 10.1038/ncomms7384, Nat Commun , 2015

Described herein is a conceptual model of the role of Pba1-Pba2 dimer during proteasome assembly. Although same binding surface is used, the dimer binds immature core particles (CPs) while the regulatory particle (RP) can bind only to the mature core particles during the assembly process. A Pall ForteBio BLItz system equipped with Ni-NTA Biosensor probe was used to capture His-tagged Pba1-Pba2. The kinetic characterization involved a serial dilution of mature CP.

Read More