MAP Kinases Bind Endothelial Nitric Oxide Synthase

Chrestensen, C.A., McMurry, J.L., Salerno, J.C., 2, 51-55 , FEBS Open Bio, 2012

Endothelial nitric oxide synthase (eNOS) is an important signaling molecule involved in control of vascular tone, insulin secretion, and angiogenesis, and contains a motif similar to recognition sequences in known MAPK binding partners. Here the Octet QK system was used to demonstrate MAP kinases ERK1/2 and p38 binding to eNOS, and characterize binding kinetics of these interactions. Also demonstrated was inhibition of p38-eNOS binding by Calmodulin. Biotinylated ERK and p38 kinases were immobilized on Streptavidin biosensors for the binding studies.

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