MAIT Recognition of a Stimulatory Bacterial Antigen Bound to MR1

López-Sagaseta J, et al., 191(10), 5268-77, J Immunol, 2013

This study reports on structural and biochemical characterization of MAIT (mucosal-associated invariant T) TCR engagement of MR1 presenting an Escherichia coli-derived stimulatory ligand, rRL-6-CH2OH. For binding analysis between humanized bovine MR1 (hbMR1) and various human MAIT TCR clones, hbMR1 expressed in the presence or absence of the E. coli supernatant was loaded onto a Ni-NTA biosensor for BLItz system studies.

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