KH Domains with Impaired Nucleic Acid Binding as a Tool for Functional Analysis

Hollingworth, D., et al., 40(14), 6873-86, Nucleic Acids Research, 2012

In this study, the authors dissect the role of K homology domains in the recognition of RNA targets by RNA-binding proteins. For binding studies, the Octet RED system was used with biotinylated RNA oligonucleotides immobilized onto Streptavidin biosensors. RNA binding constants for wild-type and mutant RNA-binding proteins were determined.

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