Isolation and Characterization of Broad and Ultrapotent Human Monoclonal Antibodies with Therapeutic Activity Against Chikungunya Virus

Smith S, et al., 18(1):86-95, Cell Host Microbe, 2015

A panel of human monoclonal antibodies isolated from a human infected with Chikungunya Virus (CHIKV) was characterized for their ability to treat immunodeficient mice inoculated with a lethal dose of CHIKV. The antigenic site for recognition by human antibodies was identified as the A domain of E2. The group of antibodies bound to the same antigenic site as revealed by a competitive binding assay performed on a Pall ForteBio Octet RED system. The CHIKV-LR2006 E2 ectodomain protein containing a polyhistidine-tag captured on to Anti-Penta-His Biosensor probes was used in the competitive binding assay.

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