Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody

Nguyen V, et al., 10(3):e0122187, PLoS One , 2015

Reported in this article is the selection and structural analyses of two specific nanobodies. Llama immunizations carried out with a purified TssM periplasmic domain allowed further understanding of an inner membrane protein called TssM from enteroaggregative Escherichia coli (EAEC). The binding characterization involved a Pall ForteBio Octet RED96 system equipped with Streptavidin (SA) Biosensor tips. Biotinylated TssJ captured onto SA tips was screened against TssM. In addition, the TssM/nanobody competition assays were also involved the Octet RED96.

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