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In Vitro Selection of Multiple Libraries Created by Genetic Code Reprogramming to Discover Macrocyclic Peptides that Antagonize VEGFR2 Activity in Living Cells

Kawakami, T, et al., 8(6), 1205-1214, ACS Chem Biol, 2013

Multiple highly diverse peptide libraries were constructed using genetic code reprogramming to incorporate non-proteinogenic amino acids. The authors used a TRAP selection scheme to select macrocyclic peptides in vitrothat bind to vascular endothelial growth factor receptor 2 (VEGFR2). Affinity of selected peptides was determined using the Octet system by immobilizing Fc-fused VEGFR2 on Anti-Human IgG Fc biosensors.

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