Impact of Gene Vector Design on the Control of Recombinant Monoclonal Antibody Production by Chinese Hamster Ovary Cells

Davies, S.L., et al., 27 (6), 1689-1699, Biotechnology Progress, 2011

In this paper, two vector design strategies for monoclonal antibody synthesis in CHO cells were compared. A dual open reading frame (ORF) expression vector utilizing separate CMV promoters to drive heavy and light chain expression independently produced higher antibody titers than a single ORF vector design employing a single promoter. Stable recombinant antibody titer from the 100 transfectants was analyzed using an Octet QK system equipped with Protein A biosensors.

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