Identifying Bottlenecks in Transient and Stable Production of Recombinant Monoclonal-antibody Sequence Variants in Chinese Hamster Ovary Cells

Mason, M., et al., 28(3), 846-855, Biotechnology Progress, 2012

The cellular mechanisms that control expression and the relationships between antibody sequence and expression level remain poorly understood. In this study, the authors examined an antibody pair in which a single amino acid substitution impacted antibody expression without altering the innate antibody function (e.g., antigen binding). Assays for antibody titer were performed on an Octet QK system with Protein A biosensors over a working range of 1-700 μg/mL.

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