Identification of the Key Functional Domain of BAFF for Binding TACI by Computer-Guided Molecular Modeling Method

Wang, R., et al., 3 (114). doi:10.4172/2157-7412.1000114, J Genet Syndr Gene Ther , 2012

In this study, the key interaction domains of BAFF (B cell activating factor) with its receptor TACI were predicted using computer-guided molecular modeling. Binding kinetics of BAFF and its mutants to TACI then were measured using the Octet RED system. Anti-Human IgG capture biosensors were loaded with TACI-Ig and incubated with various concentrations of BAFF and mutant BAFF proteins to determine kinetic rate constants and affinities.

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