Identification of Outer Membrane Porin D as a Vitronectin-binding Factor in Cystic Fibrosis Clinical Isolates of Pseudomonas Aeruginosa

Paulsson M, et al., pii: S1569-1993(15)00120-4, J Cyst Fibros, 2015

This investigation has focused on the identification of Pseudomonas aeruginosa surface receptors and their ability to bind to vitronectin. Although vitronectin is a naturally occurring plasma protein primarily involve in cell adhesion and wound healing, increased release of it by the respiratory epithelial cells suggest inflammation caused by lung diseases. A proteomics approach in conjunction with 2D SDS-PAGE allowed identification of the receptors. An outer membrane protein (OMP) called Porin D (OprD) was identified as a biding partner for vitronectin. Affinity measurements carried out by a Pall ForteBio Octet RED96 platform equipped with AR2G biosensors allowed vitronectin immobilization and KD determination.

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