Identification of Galectin-1 and Galectin-3 as Novel Partners for Von Willebrand Factor

N Saint-Lu, et al., 32(4), 894-901, Arterioscler Thromb Vasc Biol, 2012

This publication explores interactions between the heavily glycosylated von Willebrand factor (VWF) and glycan-binding proteins galectin-1 and galectin-3. The Octet QK system was used in parallel with immunosorbent microtiter plate assays for VWF-galectin binding studies. Galectin-1 and galectin-3 were coupled to Amine Reactive biosensors for the Octet platform experiments. VWF bound to immobilized galactin-1 and galactin-3 in a dose-dependent and reversible manner. The binding was significantly reduced after incubation of VWF with N-glycosidases, indicating that galectin-VWF binding is dependent on VWF glycan structures.

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