Identification of Anti-Alpha Toxin Monoclonal Antibodies that Reduce the Severity of Staphylococcus Aureus Dermonecrosis and Exhibit a Correlation Between Affinity and Potency

C Tkaczyka,, 19(3), 377-385, Clin Vaccine Immunol, 2012

Anti-alpha toxin MAbs were generated using hybridoma technology and characterized as inhibitors of alpha toxin function in Staphylococcus aureus infections. Binding kinetics and epitope competition were evaluated using Biacore and Octet systems. For epitope competition experiments, native alpha toxin was biotinylated and immobilized on Streptavidin biosensors on the Octet system.

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