High-throughput Screening and Selection of Yeast Cell Lines Expressing Monoclonal Antibodies

Barnard, G.C., et al., 37(9),961-971, J Ind Microbiol Biotechnol, 2010

The goal of the studies reported here was to design an efficient method for identifying clones able to produce therapeutic mAbs at manufacturing scale. To this end, the authors developed and implemented an integrated high- and medium-throughput screening workflow to identify glycoengineered yeast clones that express mAbs using standard microbial fermentation equipment. They used Octet platform assays to perform high throughput titer measurements for primary screening of clones.

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