High-Throughput Kinetic Screening of Hybridomas to Identify High-Affinity Antibodies Using Bio-Layer Interferometry

Lad L, et al., 20(4):498-507, J Biomol Screen, 2015

In this study authors have compared a traditional solid-phase enzyme-linked immunosorbent assay (ELISA) against Bio-Layer Interferometry (BLI) to screen high-affinity antibodies from hybridoma clones. A Pall ForteBio Octet RED384 instrument was used as their kinetic screening platform. This instrument was equipped with anti-mouse immunoglobulin G (IgG) Fc (AMC)-coated biosensor tips which was used to capture atibodies from hybridoma supernatants. This article also discusses about the advantages and limitations of different methods and platforms available for screening antobodies such as the flow cytometry, ELISA, electrochemiluminescence using Meso Scale Discovery (MSD) platform, and homogeneous time-resolved fluorescence (HTRF). Overall results suggest that the kinetic screening is an efficient method for identifying high-affinity antibodies.

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