Heterosubtypic Antibody Recognition of the Influenza Virus Hemagglutinin Receptor Binding Site Enhanced by Avidity

P.S. Lee, et al., 109(42), 17040-5, Proc Natl Acad Sci USA, 2012

Using the Octet RED system, Kd values for S139/1 Fab and IgG binding to various influenza HA strains were determined to assess enhanced virus neutralization effects due to avidity of full IgG vs. Fab. Biotinylated HA and Streptavidin biosensors were used for these measurements. The increased avidity of IgG binding was shown to reduce Kd values and improve ability of S139/1 to recognize different heterosubtypes of the virus. The Kd data correlated with neutralization data.

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