Helicobacter Pylori Hydrogenase Accessory Protein HypA and Urease Accessory Protein UreG Compete with Each Other for UreE Recognition

Benoit, S.L., et al., 1820 (10), 1519-1525, Biochimica et Biophysica Acta (BBA), 2012

In this study, BLI and SPR are used to characterize the interactions of accessory proteins implicated in the survival of H. pylori in human stomach mucosa. Proteins studied include the urease accessory proteins UreE, UreF, UreG and UreH, and the hydrogenase accessory proteins HypA and HypB. BLI protein-protein interaction measurements were performed on the Octet QK system using biotinylated accessory proteins immobilized on Streptavidin biosensors. Binding constants for two important binding pairs were determined: HypA-UreE and UreE-UreG.

Read More