Glycan-dependent and Independent Interactions Contribute to Cellular Substrate Recruitment by Calreticulin

Wijeyesakere S, Rizvi S and Raghavan M, 288(49), 35104-35116, J Biol Chem, 2013

The authors investigated different modes of calreticulin-substrate binding, and report that calreticulin binds glycosylated and non-glycosylated proteins with similar affinities but distinct kinetics and P-domain conformations. The Octet RED system and Streptavidin biosensors were used to characterize calreticulin interactions with biotin-labeled glycosylated and non-glycosylated substrates.

Read More