Generation of Stable Cell Clones Expressing Mixtures of Human Antibodies

de Kruif, J., et al., 106 (5), 741-750, Biotechnology and Bioengineering, 2010

PerC6 mammalian cells were transfected to generate stable clones expressing mixtures of human antibodies. Total human IgG was quantified using the Octet QK system using Protein A biosensors. Constant ratios of specific antibodies were produced, and total IgG productivity was also similar, averaging about 15 and 20 pg/cell/day in most subclones.

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