Generation of a Highly Diverse Panel of Antagonistic Chicken Monoclonal Antibodies Against the GIP Receptor

Konitzer J, et al., 9(3):536-549, Mabs, 2016

Chickens are emerging as crucial immunization hosts for therapeutic antibody discovery, especially in the case of difficult targets such as G-protein coupled receptors (GPCRs). Described herein is the generation of a highly diverse panel of antagonistic chicken monoclonal antibodies that are reactive against glucose-dependent insulinotropic polypeptide (GIP). A Pall Fortebio Octet HTX system equipped with Streptavidin (SA) biosensor probes were used to preform Epitope Binning experiments. In each characterization, biotinylated target (human-GIPR ECD) was captured onto SA tips and then saturated with anti-GIPR antibodies as first step. Subsequently all antibodies were tested for their ability to bind the GIPR-antibody1 complex. Distinct epitopes were identified by observing the additional binding in the second step. Overall results were reported as a 2D matrix. The data suggest chickens as superior hosts for GIPR antibody expression due to higher frequency of functionality and broader diversity.

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