Furin-cleaved Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Is Active and Modulates Low Density Lipoprotein Receptor and Serum Cholesterol Levels

Lipari, M.T., et al., 287(52), 43482-91, J Biol Chem, 2012

PCKS9 regulates plasma LDL cholesterol levels by regulating the degradation of LDL receptors. This publication investigates how cleavage of PCSK9 by furin affects PCSK9 function. LDL receptor binding to PCSK9 was characterized using the Octet RED384 system. Biotinylated LDL receptor (ectodomain) was loaded onto Streptavidin biosensors for these experiments. Kinetic binding constants for LDL receptor interaction with intact and cleaved PCSK9 are reported.

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