Functional Label-Free Assays for Characterizing the In Vitro Mechanism of Action of Small Molecule Modulators of Capsid Assembly

Lad L, et al., 54(13):2240-8, Biochemistry, 2015

A series of label-free assays were reported that would help dissecting the mechanism of action of small-molecule and peptide antiviral agents developed against the HIV capsid protein (CA). In addition to distinct assay development advantages of the Octet platform, this study has provided yet another testimony to the comparable sensitivity of the Octet platform towards small-molecule and peptide detection and binding characterization. A Pall ForteBio Octet RED384 system equipped with Super Streptavidin Biosensor probes, was used to capture biotinylated wild type CA and hexameric CA.

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