FRET-based System for Probing Protein-protein Interactions between σR and RsrA from Streptomyces Coelicolor in Response to the Redox Environment

Wei ZH, et al., 9(3):e92330, PLoS One, 2014

The authors examined the protein-protein interaction between sR and RsrA, two key analytes that trigger the cellular redox response effected by zinc binding and response to peroxide street (ROS) in the Gram Positive bacterium S. coelicolor. The Octet QK system was used to examine and confirm the binding kinetics of two proteins which were further characterized by fluorescence resonance energy transfer (FRET) studies. Streptavidin biosensors were used.

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