Following Natures Lead: On the Construction of Membrane-Inserted Toxins in Lipid Bilayer Nanodiscs

Akkaladevi N, et al., 248(3):595-607, J Membr Biol, 2015

The article highlights the use of BLI (or the BLItz system in particular) to demonstrate important prerequisites such as the immobilization, orientation, the state of folding (unfolding/refolding), and solution kinetics that make cellular entry of toxins feasible. A clear understanding of these steps is crucial for the generation of pure membrane-inserted toxins in nanodiscs for electron microscopy and for the rational design of inhibitors that target toxin membrane-insertion transitions.

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