Feasibility Study of Semi-selective Protein Precipitation with Salt-tolerant Copolymers for Industrial Purification of Therapeutic Antibodies

Capito, F, et al., doi: 10.1002/bit.24950, Biotechnol Bioeng, 2013

This publication presents a feasibility study for the use of salt-tolerant copolymers for secondary clarification of mAbs in clarified cell culture fluid. The Octet RED system was used to compare binding of mAbs before and after precipitation and redissolution. Anti-Human Fc Capture biosensors were loaded with mAbs for these measurements.

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