Exploring the Dynamic Range of the Kinetic Exclusion Assay in Characterizing Antigen-Antibody Interactions

Bee, C., et al., 7(4), e36261, PLoS One , 2012

The binding interactions of Anti-DKK (DS4) antibody to a panel of DKK Ags was characterized. The Octet QK384 syste, was used to determine the activities of various DKK proteins in different preps (carrier-free vs. lyphilized) by conducting stoichiometry-controlled titrations. These values were used to correct apparent ka values obtained by other orthogonal methods.

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