Exploiting Nucleotide Composition to Engineer Promoters

Grabherr, M.G., et al., 6(5), e20136, PLoS ONE, 2011

Artificial promoters that provide stable expression across cell lines and can be designed to the desired strength constitute an alternative to the use of viral promoters. This study demonstrates how the nucleotide characteristics of highly active human promoters can be modelled via the genome-wide frequency distribution of short motifs. The Octet QK system was used to characterize binding of the transcription factors TFIIB and TBP to artificial promoter constructs. Streptavidin biosensors were loaded with 5'-biotinylated DNA fragments of various promoter constructs for these experiments.

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