Exploiting Light Chains for the Scalable Generation and Platform Purification of Native Human Bispecific IgG

Fischer N, et al., doi: 10.1038/ncomms7113, Nat Commun, 2015

This article describes a novel platform to generate fully human bispecific IgG antibodies (BiAbs) that are unmodified and compatible with large scale manufacturing/purification processes. It uses in vitro display libraries to isolate candidates with the same heavy chain and carrying either κ or λ light chains in order to possess different target specificities. A Pall ForteBio Octet RED96 system was used to obtain the affinities and other kinetic parameters. Depending on the nature of ligand under consideration, two types of biosensor chemistries (anti-human Fc capture or Streptavidin) were employed. Biosensor tips were preconditioned/regenerated using a solution of 10mM glycin at pH 1.7.

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