Envelope Variants Circulating as Initial Neutralization Breadth Developed in Two HIV-infected Subjects Stimulate Multiclade Neutralizing Antibodies in Rabbits

Malherbe D, et al., 88(22):12949-67, J Virol, 2014

Based on the hypothesis that the B cells are developing broad antibodies by exposure to ever evolving viral envelope population, the authors tested the ability to use multiple envelops from two different subjects who developed neutralization breadth within a few years of HIV infection. They compared several different combinations of envelops from each subject to determine the most effective regimens. This approach revealed more-potent antibodies and they are modestly cross neutralizing. Surface plasmon resonance (SPR) and biolayer interferometry (BLI) techniques were used in the antigenic characterization of gp140 trimers as well as in the binding analyses of polyclonal antibodies. Anti-human IgG Fc capture (AHC) biosensors were used to capture different antibodies during the antigenic characterization. On the other hand, SA sensors were used to capture biotinylated SF162 gp140 trimer during the binding analyses.

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