Engineering HIV Envelope Protein to Activate Germline B Cell Receptors of Broadly Neutralizing Anti-CD4 Binding Site Antibodies

McGuire, A T, et al. , 210(4), 655-663, J Exp Med, 2013

The authors show that elimination of specific N-linked glycosylation sites on HIV envelope glycoprotein (Env) allows Env binding to, and activation of, B cells expressing the germline-reverted BCRs of two broadly neutralizing antibodies. Env binding studies were performed on the Octet QKe system with IgG's immobilized on Anti-Human IgG Fc Capture biosensors and wild type/mutant recombinant trimeric Env gp140.

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